This study assessed the understanding and acceptability of cultured meat among knowledgeable consumers and the public. A survey was conducted with 204 food scientists and 386 public participants to examine whether awareness of meat and food production influences cultured meat adoption. Most respondents were female (53%), meat eaters (78%), and preferred red meat (55%). Most were uncertain about the healthiness (54%) and long-term effects (71%) of cultured meat, with 82% believing it is less healthy than regular meat and 92% perceiving it as unnatural. Concerns included its impact on meat quality (71%), safety (87%), environmental effects (35%), and carbon footprint (70%). Additionally, 71% disagreed that it would improve animal welfare. Many felt scientific evidence on its safety is lacking (70%) and that it may be harmful to human health (68%). Media bias was also a concern, with 42% noting skewed portrayals and 86% believing benefits are overstated. A majority (64%) feared a rapid transition could be risky, and 91% doubted it would replace conventional beef. These findings highlight the need for further research and public engagement to address uncertainties and ensure informed participation in the future of food production.